General Operation Troubleshooting | 185
Problem Possible Cause What To Do
Protocol requires
optimization.
Optimize protocols to improve evacuation on page 40.
Aspirate tubes are
bent.
Contact BioTek TAC.
Fluid Delivery
Problem Possible Cause What To Do
Unable to
dispense
fluid.
Clogged fluid filter. Clean the fluid filter inside the supply bottle.
Inlet tube not connected. Make sure all tubing is connected properly.
Check all external tubing for kinks or clogs.
Clogged valve Create a protocol with several small primes, e.g.
10 mL, to try to unclog valve.
Clogged dispense tubes on
the washer manifold.
Remove and clean the washer manifold on page
131
No wash or rinse fluid. Fill bottles with appropriate fluid. Ensure bottles
are clean and do not contain particles or organic
material.
Unable to
dispense
fluid.
System not primed. Large
air pockets in tubing.
Run W-DAY_RINSE.
Insufficient suction,
clogged tubing, or faulty
valve.
Perform Washer Maintenance; If the problem
persists, contact BioTek TAC.
Plate
overfills
(floods).
Dispense height too high.
The aspirate tubes are too
far above the wells to
prevent overflow.
Lower the dispense height (Z-axis position) in the
protocol.
Dispense flow rate too
low.
Define a higher dispense Flow Rate in the
protocol.
Cell wash flow rate 1 or 2
is used with 384-well
plates.
Specify a non-CW dispense Flow Rate when using
384-well plates.
Aspiration tubes hit
bottom of trough during
Prime or Maintenance.
Manifold may not be properly seated or mounted.
Contact BioTek TAC.
BioTek Instruments, Inc.
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