186 | Chapter 7: Troubleshooting
Problem Possible Cause What To Do
In-line vacuum filter
plugged.
Replace or remove the in-line vacuum filter.
Loose covers on waste
bottles.
Firmly tighten waste bottle covers.
Dispense rate too fast for
volume selected.
Specify slower dispense Flow Rate or lower
volume.
Faulty vacuum pump. Contact BioTek TAC.
Insufficient or no vacuum. Firmly seat the waste bottle covers.
Check all external tubing for kinks or clogs.
When the program begins, you should be able to
hear the vacuum pump turn on. If it is not turning
on, contact BioTek TAC.
If the vacuum pump turns on, remove the
vacuum tubing from the back of the instrument
and put your finger over the port. If there is no
vacuum, contact BioTek TAC.
Uneven
dispensing
of fluid;
wells not
filled.
Clogged dispense tubes on
the washer manifold.
Remove and clean the washer manifold on page
131
Manifold or tubing not
adequately primed.
Run W-DAY_RINSE once or twice.
Dispense flow rate too
low. Flow rate 1 or 2 CW is
used with 384-well plates.
Define a higher dispense Flow Rate.
Microplate aspiration
height adjustment too high
or too low.
Change the aspirate height (Z-axis position) in
the protocol.
Fluid Leakage
Problem Possible Cause What To Do
Fluid leaking from
manifold.
Defective seals. Contact BioTek TAC
Aspiration tubes
only: vacuum too
low.
Check waste connector tubes; make sure they
are properly connected.
If you are using an in-line vacuum filter,
check the filter for clogging, and replace if
necessary.
Check seal of waste bottle covers.
Check for air leaks in the waste tubing and
405™ Microplate Washer LS
To Next Page
Loading...
