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Spectroquant Pharo 300 - Page 250

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Add 6.0 ml of B-6 with
pipette, close with the
screw cap, and mix.
Reaction time:
2 minutes
Transfer the solution
into a cell.
Select method with
AutoSelector.
Place the cell into the
cell compartment.
Pipette 5.0 ml of the
sample in to a test tube
with screw cap. (Impor-
tant: Do not use test
tubes made of glass
containing boron!)
Add 1.0 ml of B-1 with
pipette, close with the
screw cap, and mix.
Add 1.5 ml of B-2 with
pipette and close with
the screw cap.
Shake the tube
vigorously for 1 minute.
Aspirate 0.5 ml of the
clear lower phase from
the tube with pipette.
Measuring 0.050 0.800 mg/l B 10-mm cell
range: Expression of results also possible in mmol/l.
Boron
114839
Test
Transfer the extract to a
separate fresh tube.
Add 0.80 ml of B-3 with
pipette, close with the
screw cap, and mix.
Add 4 drops of B-4,
close with the screw
cap, and mix.
Add 15 drops of B-5,
close with the screw
cap, and mix.
Reaction time:
12 minutes
Quality assurance:
To check the measurement system (test reagents,
measurement device, and handling) ready-f
or-use
boron standard solution Certipur
®
, Cat.No. 119500,
concentration 1000 mg/l B can also be used after
diluting accordingly.
Check the pH of the
sample, specified range
:
pH 1 – 13.
Release 06/2014 - Spectroquant
®
UV/VIS Spectrophotometer Pharo 300

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