Pipette 0.10 ml of the
sample into a test tube.
Add 4 drops of Ca-2
and mix.
Measuring 10 – 160 mg/l Ca 14 – 224 mg/l CaO 25 – 400 mg/l CaCO
3
10-mm cell
range: 5 – 80 mg/l Ca 7 – 112 mg/l CaO 12 – 200 mg/l CaCO
3
20-mm cell
1.0 – 15.0 mg/l Ca 1.4 – 21.0 mg/l CaO 2.5 – 37.5 mg/l CaCO
3
10-mm cell
Expression of results also possible in mmol/l.
Calcium
114815
Test
Add 4 drops of Ca-3
and mix.
Reaction time:
8 minutes, measure
immediately.
Transfer the solution into
a corresponding cell
Select method with
AutoSelector measuring
range 5 - 160 mg/l Ca.
Place the cell into the
cell compartment.
Quality assurance:
To check the measurement system (test reagents,
measurement device, and handling) ready-f
or-use
calcium standard solution Certipur
®
, Cat.No. 119778,
concentration 1000 mg/l Ca, can be used after
diluting accordingly.
Add 5.0 ml of Ca-1 with
pipette and mix.
Check the pH of the
sample, specified range:
pH 4 – 10.
If required, add dilute
sodium hydroxide
solution or hydrochloric
acid drop by drop to
adjust the pH.
Release 06/2014 - Spectroquant
®
UV/VIS Spectrophotometer Pharo 300
Measuring range: 5 – 160 mg/l Ca
Pipette 0.50 ml of the
sample into a test tube.
Check the pH of the
sample, specified range:
pH 4 – 10.
If required, add dilute
sodium hydroxide
solution or hydrochloric
acid drop by drop to
adjust the pH.
Measuring range: 1.0 – 15.0 mg/l Ca
Continue as mentioned above;
starting from the addition of Ca-1 (Fig. 3). Measure
in a 10-mm cell and select method with AutoSelector
measuring range 1.0 – 15.0 mg/l Ca.
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