Chlorophyll
Determination of chlorophyll-a and phaeophytin-a
corresponds to DIN 38412 and ISO 10260
Application
Release 06/2014 - Spectroquant
®
UV/VIS Spectrophotometer Pharo 300
Transfer the solution into
a corresponding cell.
Sufficiently homogenize
0.5 - 2 l of sample. Note
the sample volume.
Filter the sample through
a suitable filter (e. g.
glass-fibre filter).
Fold the loaded filter and
tear into small pieces.
Place the pieces of the
filter in an extraction ves-
sel (e. g. 100-ml amber
glass bottle).
Add approx. 30 ml of
boiling ethanol (w = 90 %)
and allow to cool to room
temperature.
Disintegrate the filter in
the homogenizer. Rinse
together with a small
portion of ethanol.
Allow to stand for 6 - 24
hours for the extraction
to take place.
Filter the extract protect-
ed from light through a
paper filter (
“Blauband”
)
into a volumetric flask
(for DIN 38412: 100 ml).
Rinse the filter with a
small portion of ethanol.
Make the contents of the
volumetric flask up to
the mark with ethanol,
keeping them protected
from light in the process!
Measuring depending on the ratio of original sample to extract 10-mm cell Method No. 2509
range: in µg/l Chl-a or Phaeo 20-mm cell Method No. 2510
50-mm cell Method No. 2511
Attention! The measurement is carried out in a corresponding rectangular cell against a blank, prepared from
ethanol (w = 90 %).
Place the cell into the
cell compartment.
Vol (sample) [l]
Vol (extr.) [ml]
A (before acid.) [Abs]
A
(before acid.)
[Abs]
Select method no. 2509,
2510, or 2511
.
Enter the volumes of the
original sample and
extract (volumetric flask).
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