Chlorophyll
Determination of chlorophyll-a and phaeophytin-a
corresponds to DIN 38412 and ISO 10260
Application
Release 06/2014 - Spectroquant
®
UV/VIS Spectrophotometer Pharo 300
Important:
The exact procedure as well as further details on the
method used can be found in the corresponding applica-
tion.
This application can be downloaded directly at
www.analytical-test-kits.com.
To differentiate the chloro-
phyll-a content and for
the determination of the
phaeophytin-a content,
acidify a portion of the
extract with hydrochlo-
ric acid 2 mol/l Titripur
®
(Cat. No. 109063) (0.3 ml
per 100 ml of extract).
Transfer the solution into
a corresponding cell.
Place the cell into the
cell compartment a
nd
measure anew.
Differentiation
(chlorophyll-a - phaeophytin-a):
Vol (sample) [l]
Vol (extr.) [ml]
A (before acid.) [Abs]
A (after acid.) [Abs]
Chl-a [µg/l]
Chl-a [µg/l]
Vol (sample) [l]
Vol (extr.) [ml]
A (before acid.) [Abs]
A (after acid.) [Abs]
Chl-a [µg/l]
Phaeo [µg/l]
Phaeo [µg/l]
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