Spectroquant Pharo 300 - Page 272

Chlorophyll
Determination of chlorophyll-a and phaeophytin-a
analogous to APHA 10200-H
Application
Release 06/2014 - Spectroquant
®
UV/VIS Spectrophotometer Pharo 300
Place the cell into the
cell compartment.
Transfer the solution into
a corresponding cell.
Disintegrate the filter in
the homogenizer.
Allow to stand at +4 °C
for at least 2 hours for
the extraction to take
place.
Filter the extract protect-
ed from light through a
suitable filter.
Vol (sample) [l]
Vol (extr.) [ml]
A (before acid.) [Abs]
A (before acid.)
[Abs]
Make up to 10 ml
with extracting agent.
Select method no. 2504,
2505, or 2506
.
Enter the volumes of the
original sample and
extract (here: 10 ml).
Measuring depending on the ratio of original sample to extract 10-mm cell Method No. 2504
range: in mg/m
3
Chl-a or Phaeo-a 20-mm cell Method No. 2505
50-mm cell Method No. 2506
Attention! The measurement is carried out in a corresponding rectangular cell against a blank, prepared from
extracting agent.
Sufficiently homogenize
the sample. Note the
sample volume.
Filter the sample through
a suitable filter (e. g.
glass-fibre filter).
Fold the loaded filter and
tear into small pieces.
Place the pieces of the
filter in an extraction ves-
sel (protected from
light).
Add 2 - 3 ml of
extracting agent.