Chlorophyll
Determination of chlorophyll-a and phaeophytin-a
analogous to ASTM D3731 - 87
Application
Transfer the solution into
a corresponding cell.
Vol (sample) [l]
Vol (extr.) [ml]
A (before acid.) [Abs]
A (after acid.) [Abs]
Chl-a [mg/m
3
]
Chl-a [mg/m
3
]
Vol (sample) [l]
Vol (extr.) [ml]
A (before acid.) [Abs]
A (after acid.) [Abs]
Chl-a [mg/m
3
]
Phaeo-a [mg/m
3
]
Phaeo-a [mg/m
3
]
Important:
The exact procedure and the composition and preparation
of the extraction agent used are giv
en in the correspond-
ing application, which also includes further information on
the method employed. This application can be download-
ed directly at www.analytical-test-kits.com.
To differentiate the chloro-
phyll-a content and for
the determination of the
phaeophytin-a content,
acidify a portion of the
extract with hydrochlo-
ric acid 1 mol/l
Titripur
®
(Cat. No.
109057) (50 µl per
5 ml of extract).
Place the cell into the
cell compartment a
nd
measure anew.
Differentiation
(chlorophyll-a - phaeophytin-a):
Release 06/2014 - Spectroquant
®
UV/VIS Spectrophotometer Pharo 300
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