Chlorophyll-a, -b, -c
(Trichromatic Method)
analogous to APHA 10200-H
Application
Place the cell into the
cell compartment.
Vol (sample) [l]
Vol (extr.) [ml]
Chl-a [Abs]
Chl-a [mg/m
3
]
Chl-a [mg/m
3
]
Vol (sample) [l]
Vol (extr.) [ml]
Chl-a [Abs]
Chl-a [mg/m
3
]
Chl-b [mg/m
3
]
Chl-b [mg/m
3
]
Vol (sample) [l]
Vol (extr.) [ml]
Chl-a [Abs]
Chl-a [mg/m
3
]
Chl-b [mg/m
3
]
Chl-c [mg/m
3
]
Chl-c [mg/m
3
]
Select method no. 2507
or 2508
.
Enter the volumes of the
original sample and
extract (here: 10 ml).
Release 06/2014 - Spectroquant
®
UV/VIS Spectrophotometer Pharo 300
Important:
The exact procedure and the composition and preparation
of the extraction agent used are giv
en in the correspond-
ing application, which also includes further information on
the method employed. This application can be download-
ed directly at www.analytical-test-kits.com.
Transfer the solution into
a corresponding cell.
Disintegrate the filter in
the homogenizer.
Allow to stand at +4 °C
for at least 2 hours for
the extraction to take
place.
Filter the extract protect-
ed from light through a
suitable filter.
Make up to 10 ml
with extracting agent.
Measuring depending on the ratio of original sample to extract 10-mm cell Method No. 2507
range: in mg/m
3
Chl-a, -b, -c 50-mm cell Method No. 2508
Attention! The measurement is carried out in a corresponding rectangular cell against a blank, prepared from
extracting agent.
Sufficiently homogenize
the sample. Note the
sample volume.
Filter the sample through
a suitable filter (e. g.
glass-fibre filter).
Fold the loaded filter and
tear into small pieces.
Place the pieces of the
filter in an extraction ves-
sel (protected from
light).
Add 2 - 3 ml of
extracting agent.
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