Spectroquant Pharo 300 - Page 302

114553
Cell Test
Copper
Quality assurance:
To check the measurement system (test reagents,
measurement device, and handling) w
e recommended
to use Spectroquant
®
CombiCheck 30, Cat.No. 114677.
Ready-for-use copper standard solution Certipur
®
,
Cat.No. 119786, concentration 1000 mg/l Cu, can also
be used after diluting accordingly.
To check for sample-dependent effects the use of addition
solutions (e.g. in CombiCheck 30) is highly recommended.
Important:
Very high copper concentrations in the sample produce
turquoise-colored solutions (measurement solution should
be blue) and false-lo
w readings are yielded.
In such cases the sample must be diluted (plausibility
check).
For the determination of total copper a pretreatment
with
Crack Set 10C, Cat.No. 114688, or Crack Set 10,
Cat.No. 114687 and thermoreactor is necessary.
Result can be expressed as sum of copper ( Cu).
Measuring 0.05 – 8.00 mg/l Cu
range: Expression of results also possible in mmol/l.
Pipette 5.0 ml of the
sample into a reaction
cell, close with the screw
cap, and mix.
Add 5 drops of Cu-1K,
close the cell with the
screw cap, and mix.
Place the cell into the
cell compartment. Align
the mark on the cell with
that on the photometer.
Reaction time:
5 minutes
Check the pH of the
sample, specified range:
pH 4 – 10.
If required, add dilute
sodium hydroxide
solution or sulfuric acid
drop by drop to adjust
the pH.
Release 06/2014 - Spectroquant
®
UV/VIS Spectrophotometer Pharo 300