Spectroquant Pharo 300 - Page 307

Add 1 dose of CN-1K
using the green dose-
metering cap, close the
cell with the screw cap.
Measuring 0.010 – 0.500 mg/l CN
range: Expression of results also possible in mmol/l and cyanide readily liberated [CN(v)].
Cyanide
Determination of readily liberated cyanide
114561
Cell Test
Reaction time:
10 minutes
Place the cell into the
cell compartment. Align
the mark on the cell with
that on the photometer.
Pipette 10 ml of the
sample into an empty
round cell
(Empty cells,
Cat.No. 114724).
Heat the cell in the
thermoreactor at 120 °C
(100 °C) for 30 minutes.
Remove the cell from
the thermoreactor and
place in a test-tube rack
to cool to room tempera-
ture.
Swirl the cell before
opening.
Add 3 drops of CN-2K,
close with the screw cap,
and mix: pretreated
sample.
Pipette 5.0 ml of the
pretreated sample
into a reaction cell , close
with the screw cap,
and
dissolve the solid sub-
stance.
Add 1 level blue micro-
spoon of CN-3K, close
the cell with the screw
cap.
Shake the cell vigorous-
ly to dissolve the solid
substance.
Quality assurance:
To check the measurement system (test reagents,
measurement device, and handling) ready-f
or-use
cyanide standard solution Certipur
®
, Cat.No. 119533,
concentration
1000 mg/l CN
–
, can be used after diluting
accordingly.
Check the pH of the
sample, specified range
:
pH 4.5 – 8.0.
If required, add dilute
sodium hydroxide
solution or sulfuric acid
drop by drop to adjust
the pH.
Release 06/2014 - Spectroquant
®
UV/VIS Spectrophotometer Pharo 300