Spectroquant Pharo 300 - Page 308

Measuring 0.010 – 0.500 mg/l CN 10-mm cell
range: 0.005 – 0.250 mg/l CN 20-mm cell
0.0020 – 0.1000 mg/l CN 50-mm cell
Expression of results also possible in mmol/l and cyanide free [CN(f)].
Cyanide
Determination of free cyanide
109701
Test
Pipette 5.0 ml of the
sample into an empty
round cell (Empty cells,
Cat.No. 114724).
Add 1 level green micro-
spoon of CN-3, close the
cell with the screw cap.
Reaction time:
10 minutes
Transfer the solution into
a corresponding rectan-
gular cell.
Select method with
AutoSelector.
Place the cell into the
cell compartment.
Shake the cell vigorous-
ly to dissolve the solid
substance.
Add 1 level blue micro-
spoon of CN-4, close the
cell with the screw cap.
Shake the cell vigorous-
ly to dissolve the solid
substance.
Quality assurance:
To check the measurement system (test reagents,
measurement device, and handling) ready-f
or-use
cyanide standard solution Certipur
®
, Cat.No. 119533,
concentration
1000 mg/l CN
–
, can be used after diluting
accordingly.
Check the pH of the
sample, specified range
:
pH 4.5 – 8.0.
If required, add dilute
sodium hydroxide
solution or sulfuric acid
drop by drop to adjust
the pH.
Note:
Empty cells with screw caps, Cat.No. 114724 are
recommended for the preparation.
These cells can be
sealed with the screw caps, thus preventing any gas
losses.
Release 06/2014 - Spectroquant
®
UV/VIS Spectrophotometer Pharo 300
Important:
To measure in the 50-mm cell, the sample volume and
the volume of the reagents CN-3 and CN-4 hav
e to be
doubled for each.
Alternatively, the semi-microcell, Cat.No. 173502, can be
used.