Spectroquant Pharo 300 - Page 311

114557
Cell Test
Measuring 0.10 – 1.50 mg/l F Round cell
range: 0.025 – 0.500 mg/l F 50-mm cell (see “sensitive” preparation procedure)
Expression of results also possible in mmol/l.
Fluoride
Swirl the cell before
measurement.
Quality assurance:
To check the measurement system (test reagents,
measurement device, and handling) ready-f
or-use
fluoride standard solution Certipur
®
, Cat.No. 119814,
concentration 1000 mg/l F
–
, can be used after diluting
accordingly.
Check the pH of the
sample, specified range:
pH 3 – 8.
If required, add dilute
sodium hydroxide
solution or sulfuric acid
drop by drop to adjust
the pH.
Pipette 5.0 ml of the
sample into a reaction
cell, close with the screw
cap, and mix.
Add 1 dose of F-1K
using the blue dose-
metering cap, close the
cell with the screw cap.
Shake the cell vigorous-
ly to dissolve the solid
substance.
Reaction time:
5 minutes
Fluoride sensitive
Use the same preparation procedure as above, but
add 10 ml of sample instead of 5.0 ml. Prepare an
own blank by using 10 ml of distilled water and all
reagents. For measurement transfer the solution into
a 50-mm cell. Select method F sens in the menu
(method no. 124).
Place the cell into the
cell compartment. Align
the mark on the cell with
that on the photometer.
Important:
Very high fluoride concentrations in the sample produce
brown-colored solutions (measurement solution should be
violet) and false-lo
w readings are yielded. In such cases
the sample must be diluted (plausibility check).
Release 06/2014 - Spectroquant
®
UV/VIS Spectrophotometer Pharo 300