Measuring 1.0 – 50.0 mg/l Fe
range: Expression of results also possible in mmol/l and also in Fe(II), Fe(III).
Pipette 1.0 ml of the
sample into a reaction
cell, close with the screw
cap, and mix.
Shake the cell vigorous-
ly to dissolve the solid
substance.
Quality assurance:
To check the measurement system (test reagents,
measurement device, and handling) ready-f
or-use
iron standard solution Certipur
®
, Cat.No. 119687,
concentration 1000 mg/l Fe(III), can be used after
diluting accordingly.
Important:
For the determination of total iron a pretreatment
with Cr
ack Set 10C, Cat.No. 114688, or Crack Set 10,
Cat.No. 114687, and thermoreactor is necessary.
Result can be expressed as sum of iron (
Fe).
Determination of iron (II)
114896
Cell Test
Iron
Determination of iron(II) and iron(III)
Reaction time:
5 minutes
Place the cell into the
cell compartment. Align
the mark on the cell with
that on the photometer.
Determination of iron (II + III)
Pipette 1.0 ml of the
sample into a reaction
cell
, close with the
screw cap,
and mix.
Reaction time:
5 minutes
Place the cell into the cell
compartment. Align the
mark on the cell with that
on the photometer.
Add 1 dose of Fe-1K
using the blue dose-
metering cap, close the
reaction cell with the
screw cap.
A differentiation between iron(II) and iron(III)
can be performed on the photometer. Prior to
measuring, select the differentiation meas-
urement and choose the corresponding
citation form.
Then measure the iron(II + III) (result for “Fe
total” is shown on the display), press enter
and measure the iron(II). T
he individual mea-
suring values for Fe II and Fe III are shown on
the display.
Check the pH of the
sample, specified range:
pH 3 – 8.
If required, add dilute
sodium hydroxide
solution or hydrochloric
acid drop by drop to
adjust the pH.
Check the pH of the
sample, specified range:
pH 3 – 8.
If required, add dilute
sodium hydroxide
solution or hydrochloric
acid drop by drop to
adjust the pH.
Release 06/2014 - Spectroquant
®
UV/VIS Spectrophotometer Pharo 300
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