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Spectroquant Pharo 300 - Page 327

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Pipette 5.0 ml of the
sample into a test tube.
Add 3 drops of Fe-1 and
mix.
Reaction time:
3 minutes
Quality assurance:
To check the measurement system (test reagents,
measurement device, and handling) w
e recommended
to use Spectroquant
®
CombiCheck 30, Cat.No. 114677.
Ready-for-use iron standard solution Certipur
®
,
Cat.No. 119781, concentration 1000 mg/l Fe, can also
be used after diluting accordingly.
To check for sample-dependent effects the use of addition
solutions (e.g. in CombiCheck 30) is highly recommended.
Important:
For the determination of total iron a pretreatment
with Cr
ack Set 10C, Cat.No. 114688, or Crack Set 10,
Cat.No. 114687 and thermoreactor is necessary.
Result can be expressed as sum of iron (
Fe).
To measure in the 50-mm cell, the sample volume and
the volume of the reagents have to be doubled for each.
114761
Test
Measuring 0.05 5.00 mg/l Fe 10-mm cell
range: 0.03 2.50 mg/l Fe 20-mm cell
0.005 1.000 mg/l Fe 50-mm cell
Expression of results also possible in mmol/l.
Iron
Transfer the solution into
a corresponding cell.
Select method with
AutoSelector.
Place the cell into the
cell compartment.
Check the pH of the
sample, specified range:
pH 1 – 10.
If required, add dilute
sodium hydroxide
solution or hydrochloric
acid drop by drop to
adjust the pH.
Release 06/2014 - Spectroquant
®
UV/VIS Spectrophotometer Pharo 300

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