Pipette 8.0 ml of the
sample into a test tube.
Add 1 drop of Fe-1 and
mix.
Add 0.50 ml of Fe-2 with
pipette and mix.
Quality assurance:
To check the measurement system (test reagents,
measurement device, and handling) w
e recommended
to use Spectroquant
®
CombiCheck 30, Cat.No. 114677.
Ready-for-use iron standard solution Certipur
®
,
Cat.No. 119781, concentration 1000 mg/l Fe(III), can also
be used after diluting accordingly.
To check for sample-dependent effects the use of addition
solutions (e.g. in CombiCheck 30) is highly recommended.
Important:
For the determination of total iron a pretreatment
with Cr
ack Set 10C, Cat.No. 114688, or Crack Set 10,
Cat.No. 114687 and thermoreactor is necessary.
100796
Test
Measuring 0.10 – 5.00 mg/l Fe 10-mm cell
range: 0.05 – 2.50 mg/l Fe 20-mm cell
0.010– 1.000 mg/l Fe 50-mm cell
Expression of results also possible in mmol/l.
Iron
Determination of iron(II) and iron(III)
Add 1 dose of Fe-3
using the blue dose-
metering cap and
dissolve the solid sub-
stance.
Place the cell into the
cell compartment.
Reaction time:
10 minutes,
then measure.
Reaction time:
5 minutes
Transfer the solution into
a corresponding cell.
Select method with
AutoSelector.
Determination of iron(II)
Determination of iron(II + III)
Same preparation as dis-
cribed above. After adding
of Fe-2 continue with
Fe-3.
Check the pH of the
sample, specified range:
pH 2 – 8.
If required, add dilute
sodium hydroxide
solution or nitric acid
drop by drop to adjust
the pH.
Release 06/2014 - Spectroquant
®
UV/VIS Spectrophotometer Pharo 300
A differentiation between iron(II) and iron(III)
can be performed on the photometer. Prior to
measuring, select the differentiation meas-
urement and choose the corresponding
citation form.
Then measure the iron(II) (result for “Fe II” is
shown on the display), press enter
and mea-
sure the iron(
II + III
). T
he individual measuring
values for Fe total and Fe III are shown on the
display.
To Next Page