Spectroquant Pharo 300 - Page 348

Nitrate
109713
Test
Important:
To measure in the 50-mm cell, the sample volume and the
volume of the reagents hav
e to be doubled for each.
Alternatively, the semi-microcell, Cat.No. 173502, can be
used.
Pipette 4.0 ml of NO
3
-1
into a dry empty round
cell (Empty cells, Cat.
No. 114724).
Transfer the solution into
a corresponding rectan-
gular cell.
Select method with
AutoSelector.
Place the cell into the
cell compartment.
Add 0.50 ml of NO
3
-2
with pipette
, close the
cell with the screw cap,
and mix. Caution, cell
becomes hot!
Measuring 1.0 – 25.0 mg/l NO
3
-N 4.4 – 110.7 mg/l NO
3
10-mm cell
range: 0.5 – 12.5 mg/l NO
3
-N 2.2 – 55.3 mg/l NO
3
20-mm cell
0.10 – 5.00 mg/l NO
3
-N 0.4 – 22.1 mg/l NO
3
50-mm cell
Expression of results also possible in mmol/l.
Add 0.50 ml of the
sample with pipette
, do
not mix.
Reaction time:
10 minutes
Note:
Empty cells with screw caps, Cat.No. 114724 are
recommended for the preparation.
These cells can be
sealed with the screw caps, thus enabling a hazard-free
mixing of the sample.
Release 06/2014 - Spectroquant
®
UV/VIS Spectrophotometer Pharo 300
Quality assurance:
To check the measurement system (test reagents, meas-
urement device, and handling) w
e recommended to use
Spectroquant
®
CombiCheck 20, Cat.No. 114675, or the
Standard solution for photometric applications, CRM,
Cat.No. 125036, 125037, and 125038.
Ready-for-use nitrate standard solution Certipur
®
,
Cat.No. 119811, concentration 1000 mg/l NO
3
–
, can also
be used after diluting accordingly.
To check for sample-dependent effects the use of addition
solutions (e.g. in CombiCheck 20) is highly recommended.