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Spectroquant Pharo 300 - Page 350

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Pipette 5.0 ml of NO
3
-1
into a dry empty round
cell (Empty cells, Cat.
No. 114724).
Nitrate
in seawater
114942
Test
Transfer the solution
into a rectangular cell.
Select method with
AutoSelector.
Place the cell into the
cell compartment.
Immediately add
1.5 ml of NO
3
-2 with
pipette.
Close cell tightly and
shake vigorously.
Measuring 0.2 – 17.0 mg/l NO
3
-N 0.9 – 75.3 mg/l NO
3
10-mm cell
range: Expression of results also possible in mmol/l.
Add 1.0 ml of the
sample with pipette.
Caution, cell becomes
hot!
Reaction time:
15 minutes
Add 2 level grey micro-
spoons of NO
3
-3.
Close cell tightly and
shake vigorously until
the reagent is complete-
ly dissolved.
Important:
Empty cells with screw caps, Cat.No. 114724 are
recommended for the preparation.
These cells can be
sealed with the screw caps, thus enabling a hazard-free
mixing of the sample.
Reaction time:
60 minutes
Release 06/2014 - Spectroquant
®
UV/VIS Spectrophotometer Pharo 300
Quality assurance:
To check the measurement system (test reagents, meas-
urement device, and handling) w
e recommended to use
Spectroquant
®
CombiCheck 20, Cat.No. 114675, or the
Standard solution for photometric applications, CRM,
Cat.No. 125036, 125037, and 125038.
Ready-for-use nitrate standard solution Certipur
®
,
Cat.No. 119811, concentration 1000 mg/l NO
3
, can also
be used after diluting accordingly.
To check for sample-dependent effects the use of addition
solutions (e.g. in CombiCheck 20) is highly recommended.

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