Spectroquant Pharo 300 - Page 355

Check the pH, specified
range:
pH 2.0 – 2.5.
If required, add dilute
sodium hydroxide
solution or sulfuric acid
drop by drop to adjust
the pH.
Pipette 5.0 ml of the
sample into a test tube.
Nitrite
114776
Test
Transfer the solution into
a corresponding cell.
Select method with
AutoSelector.
Place the cell into the
cell compartment.
Important:
To measure in the 50-mm cell, the sample volume and
the volume of the reagents hav
e to be doubled for each.
Alternatively, the semi-microcell, Cat.No. 173502, can be
used.
Shake vigorously to
dissolve the solid
substance.
Measuring 0.02 – 1.00 mg/l NO
2
-N 0.07 – 3.28 mg/l NO
2
10-mm cell
range: 0.010 – 0.500 mg/l NO
2
-N 0.03 – 1.64 mg/l NO
2
20-mm cell
0.002 – 0.200 mg/l NO
2
-N 0.007 – 0.657 mg/l NO
2
50-mm cell
Expression of results also possible in mmol/l.
Add 1 level blue micro-
spoon of NO
2
-1 .
Reaction time:
10 minutes
Check the pH of the
sample, specified range
:
pH 2 – 10.
If required, add dilute
sulfuric acid drop by
drop to adjust the pH.
Release 06/2014 - Spectroquant
®
UV/VIS Spectrophotometer Pharo 300
Quality assurance:
To check the measurement system (test reagents, meas-
urement device, and handling) ready-f
or-use nitrite stan-
dard solution Certipur
®
, Cat.No. 119899, concentration
1000 mg/l NO
2
–
, can be used after diluting accordingly as
well as the Standard solution for photometric applications,
CRM, Cat.No. 125041.