Spectroquant Pharo 300 - Page 356

Pipette 10 ml of the
sample into an empty
round cell (Empty cells,
Cat.No. 114724).
Add very slowly 1.5 ml
of the pretreated
sample with pipette,
close the cell with the
screw cap, and mix
briefly.
Caution, cell becomes
hot!
Measuring 0.5 – 15.0 mg/l N
range: Expression of results also possible in mmol/l.
Reaction time:
10 minutes
Place the cell into the
cell compartment. Align
the mark on the cell with
that on the photometer.
114537
Cell Test
Nitrogen (total)
Add 1 level blue micro-
spoon of N-1K.
Add 6 drops of N-2K,
close the cell with the
screw cap, and mix.
Heat the cell in the
thermoreactor at 120 °C
(100 °C) for 1 hour.
Remove the cell from
the thermoreactor and
place in a test-tube rack
to cool to room tempera-
ture: pretreated sam-
ple.
Add 1 level yellow micro-
spoon of N-3K into a
reaction cell, close the
cell with the screw cap.
Shake the cell vigor-
ously for 1 minute
to dissolve the solid sub-
stance.
Swirl the cell after
10 minutes.
Release 06/2014 - Spectroquant
®
UV/VIS Spectrophotometer Pharo 300
Quality assurance:
To check the measurement system (test reagents, meas-
urement device, and handling) w
e recommended to use
Spectroquant
®
CombiCheck 50, Cat.No. 114695, or the
Standard solution for photometric applications, CRM,
Cat.No. 125043 and 125044.
To check for sample-dependent effects the use of addition
solutions (e.g. in CombiCheck 50) is highly recommended.