Spectroquant Pharo 300 - Page 357

Pipette 10 ml of the
sample into an empty
round cell (Empty cells,
Cat.No. 114724).
Add 6 drops of N-2K,
close the cell with the
screw cap, and mix.
Measuring 0.5 – 15.0 mg/l N
range: Expression of results also possible in mmol/l.
Reaction time:
10 minutes
Place the cell into the
cell compartment. Align
the mark on the cell with
that on the photometer.
100613
Cell Test
Nitrogen (total)
Add 1 level blue micro-
spoon of N-1K.
Heat the cell in the
thermoreactor at 120 °C
(100 °C) for 1 hour.
Remove the cell from
the thermoreactor and
place in a test-tube rack
to cool to room tempera-
ture: pretreated sam-
ple.
Pipette 1.0 ml of the
pretreated sample into
a reaction cell, do not
mix!
Add 1.0 ml of N-3K with
pipette
, close the cell
with the screw cap, and
mix.
Caution, cell be-
comes hot!
Swirl the cell after
10 minutes.
Release 06/2014 - Spectroquant
®
UV/VIS Spectrophotometer Pharo 300
Quality assurance:
To check the measurement system (test reagents, meas-
urement device, and handling) w
e recommended to use
Spectroquant
®
CombiCheck 50, Cat.No. 114695, or the
Standard solution for photometric applications, CRM,
Cat.No. 125043 and 125044.
To check for sample-dependent effects the use of addition
solutions (e.g. in CombiCheck 50) is highly recommended.