Spectroquant Pharo 300 - Page 358

Pipette 1.0 ml of the
sample into an empty
round cell.
Add 6 drops of N-2K,
close the cell with the
screw cap, and mix.
Measuring 10 –150 mg/l N
range: Expression of results also possible in mmol/l.
Reaction time:
10 minutes
Place the cell into the
cell compartment. Align
the mark on the cell with
that on the photometer.
114763
Cell Test
Nitrogen (total)
Add 9.0 ml of distilled
water (Water for analysis
EMSURE
®
, Cat.No.
116754, is recommend-
ed) with pipette.
Add 1 level blue micro-
spoon of N-1K.
Heat the cell in the
thermoreactor at 120 °C
(100 °C) for 1 hour.
Remove the cell from
the thermoreactor and
place in a test-tube rack
to cool to room tempera-
ture: pretreated sam-
ple.
Pipette 1.0 ml of the
pretreated sample into
a reaction cell, do not
mix!
Add 1.0 ml of N-3K with
pipette
, close the cell
with the screw cap, and
mix.
Caution, cell be-
comes hot!
Swirl the cell after
10 minutes.
Release 06/2014 - Spectroquant
®
UV/VIS Spectrophotometer Pharo 300
Quality assurance:
To check the measurement system (test reagents, meas-
urement device, and handling) w
e recommended to use
Spectroquant
®
CombiCheck 70, Cat.No. 114689, or the
Standard solution for photometric applications, CRM,
Cat.No. 125044 and 125045.
To check for sample-dependent effects the use of addition
solutions (e.g. in CombiCheck 70) is highly recommended.