Spectroquant Pharo 300 - Page 368

Check the pH of the
sample, specified range:
pH 0 – 10.
If required, add dilute
sulfuric acid drop by
drop to adjust the pH.
Pipette 5.0 ml of the
sample into a reaction
cell, close with the screw
cap, and mix.
Add 5 drops of P-2K,
close the cell with the
screw cap, and mix.
Shake the cell vigorous-
ly to dissolve the solid
substance.
Measuring 0.05 – 5.00 mg/l PO
4
-P
range: 0.2 – 15.3 mg/l PO
4
0.11 – 11.46 mg/l P
2
O
5
Expression of results also possible in mmol/l.
Reaction time:
5 minutes
Quality assurance:
To check the measurement system (test reagents,
measurement device, and handling) w
e recommended
to use Spectroquant
®
CombiCheck 10, Cat.No. 114676.
Ready-for-use phosphate standard solution Certipur
®
,
Cat.No. 119898,
concentration 1000 mg/l PO
4
3–
, can also
be used after diluting accordingly.
To check for sample-dependent effects the use of addition
solutions (e.g. in CombiCheck 10) is highly recommended.
114543
Cell Test
Phosphate
Determination of orthophosphate
Add 1 dose of P-3K
using the blue dose-
metering cap, close the
cell with the screw cap.
Place the cell into the
cell compartment. Align
the mark on the cell with
that on the photometer.
Release 06/2014 - Spectroquant
®
UV/VIS Spectrophotometer Pharo 300