Pipette 5.0 ml of the
sample into a reaction
cell, close with the screw
cap, and mix.
Add 5 drops of P-2K,
close the cell with the
screw cap, and mix.
Shake the cell vigorous-
ly to dissolve the solid
substance.
Reaction time:
5 minutes
Add 1 dose of P-3K
using the blue dose-
metering cap, close the
cell with the screw cap.
Place the cell into the
cell compartment. Align
the mark on the cell with
that on the photometer.
114543
Cell Test
Phosphate
Determination of total phosphorus
= sum of orthophosphate, polyphosphate, and organophosphate
Add 1 dose of P-1K
using the green dose-
metering cap, close the
cell with the screw cap.
Heat the cell in the
thermoreactor at
120 °C
(100 °C) for 30 minutes.
Remove the cell from
the thermoreactor and
place in a test-tube rack
to cool to room tempera-
ture.
* P org is the sum of polyphosphate
and organophosphate.
A differentiation between orthophosphate
(PO
4
-P) and P org* (P(o)) can be performed on
the photometer. Prior to measuring, select the
differentiation measure ment and choose the
corresponding
citation form. Then measure the
P total
(result for “P total” is shown on the dis-
play)
, press
enter and measure the ortho-phos-
phate (see ana lytical procedure for ortho-phos-
phate).
The individual measur ing
values for
PO
4
-P and P(o) are shown on the display.
Measuring 0.05 – 5.00 mg/l P
range: 0.2 – 15.3 mg/l PO
4
0.11 – 11.46 mg/l P
2
O
5
Expression of results also possible in mmol/l and also in P total ( P) and P org* [P(o)].
Check the pH of the
sample, specified range:
pH 0 – 10.
If required, add dilute
sulfuric acid drop by
drop to adjust the pH.
Release 06/2014 - Spectroquant
®
UV/VIS Spectrophotometer Pharo 300
Quality assurance:
To check the measurement system (test reagents, meas-
urement device, and handling) w
e recommended to use
Spectroquant
®
CombiCheck 10, Cat.No. 114676, or the
Standard solution for photometric applications, CRM,
Cat.No. 125046 and 125047.
Ready-for-use phosphate standard solution Certipur
®
,
Cat.No. 119898,
concentration 1000 mg/l PO
4
3–
, can also
be used after diluting accordingly.
To check for sample-dependent effects the use of addition
solutions (e.g. in CombiCheck 10) is highly recommended.
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