Spectroquant Pharo 300 - Page 371

Pipette 1.0 ml of the
sample into a reaction
cell, close with the screw
cap, and mix.
Add 5 drops of P-2K,
close the cell with the
screw cap, and mix.
Shake the cell vigorous-
ly to dissolve the solid
substance.
Measuring 0.5 – 25.0 mg/l PO
4
-P
range: 1.5 – 76.7 mg/l PO
4
1.1– 57.3 mg/l P
2
O
5
Expression of results also possible in mmol/l.
Reaction time:
5 minutes
Quality assurance:
To check the measurement system (test reagents,
measurement device, and handling) w
e recommended
to use Spectroquant
®
CombiCheck 20 and 80, Cat.No.
114675 and 114738.
Ready-for-use phosphate standard solution Certipur
®
,
Cat.No. 119898,
concentration 1000 mg/l PO
4
3–
, can also
be used after diluting accordingly.
To check for sample-dependent effects the use of addition
solutions (e.g. in CombiCheck) is highly recommended.
Add 1 dose of P-3K
using the blue dose-
metering cap, close the
cell with the screw cap.
Place the cell into the
cell compartment. Align
the mark on the cell with
that on the photometer.
114729
Cell Test
Phosphate
Determination of orthophosphate
Check the pH of the
sample, specified range:
pH 0 – 10.
If required, add dilute
sulfuric acid drop by
drop to adjust the pH.
Release 06/2014 - Spectroquant
®
UV/VIS Spectrophotometer Pharo 300