Measuring 0.5 – 25.0 mg/l P
range: 1.5 – 76.7 mg/l PO
4
1.1– 57.3 mg/l P
2
O
5
Expression of results also possible in mmol/l and also in P total ( P) and P org* [P(o)].
114729
Cell Test
Phosphate
Determination of total phosphorus
= sum of orthophosphate, polyphosphate, and organophosphate
Pipette 1.0 ml of the
sample into a reaction
cell, close with the screw
cap, and mix.
Heat the cell in the
thermoreactor at 120 °C
(100 °C) for 30 minutes.
Remove the cell from
the thermoreactor and
place in a test-tube rack
to cool to room tempera-
ture.
Add 1 dose of P-1K
using the green dose-
metering cap, close the
cell with the screw cap.
Add 5 drops of P-2K,
close the cell with the
screw cap, and mix.
Shake the cell vigorous-
ly to dissolve the solid
substance.
Reaction time:
5 minutes
Add 1 dose of P-3K
using the blue dose-
metering cap, close the
cell with the screw cap.
Place the cell into the
cell compartment. Align
the mark on the cell with
that on the photometer.
* P org is the sum of polyphosphate
and organophosphate.
A differentiation between orthophosphate
(PO
4
-P) and P org* (P(o)) can be performed on
the photometer. Prior to measuring, select the
differentiation measure ment and choose the
corresponding
citation form. Then measure the
P total
(result for “P total” is shown on the dis-
play)
, press
enter and measure the ortho-phos-
phate (see ana lytical procedure for ortho-phos-
phate).
The individual measur ing
values for
PO
4
-P and P(o) are shown on the display.
Check the pH of the
sample, specified range:
pH 0 – 10.
If required, add dilute
sulfuric acid drop by
drop to adjust the pH.
Release 06/2014 - Spectroquant
®
UV/VIS Spectrophotometer Pharo 300
Quality assurance:
To check the measurement system (test reagents, meas-
urement device, and handling) w
e recommended to use
Spectroquant
®
CombiCheck 20 and 80, Cat.No. 114675
and 114738, or as well as the Standard solution for photo-
metric applications, CRM, Cat.No. 125047 and 125048.
Ready-for-use phosphate standard solution Certipur
®
,
Cat.No. 119898,
concentration 1000 mg/l PO
4
3–
, can also
be used after diluting accordingly.
To check for sample-dependent effects the use of addition
solutions (e.g. in CombiCheck) is highly recommended.
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