Spectroquant Pharo 300 - Page 373

Pipette 0.20 ml of the
sample into a reaction
cell, close with the screw
cap, and mix.
Add 5 drops of PO
4
-1K,
close the cell with the
screw cap, and mix.
Add 1 dose of PO
4
-2K
using the blue dose-
metering cap, close the
cell with the screw cap.
Measuring 3.0 – 100.0 mg/l PO
4
-P
range: 9 – 307 mg/l PO
4
7 – 229 mg/l P
2
O
5
Expression of results also possible in mmol/l.
Reaction time:
5 minutes
Quality assurance:
To check the measurement system (test reagents,
measurement device, and handling) r
eady-for-use
phosphate standard solution Certipur
®
, Cat.No. 119898,
concentration 1000 mg/l PO
4
3–
, can be used after
diluting accordingly.
Shake the cell vigorous-
ly to dissolve the solid
substance.
Place the cell into the
cell compartment. Align
the mark on the cell with
that on the photometer.
100616
Cell Test
Phosphate
Determination of orthophosphate
Check the pH of the
sample, specified range:
pH 0 – 10.
If required, add dilute
sulfuric acid drop by
drop to adjust the pH.
Release 06/2014 - Spectroquant
®
UV/VIS Spectrophotometer Pharo 300
Important:
For the determination of total phosphorus = sum of
orthophosphate,
polyphosphate and organophosphate
either Phosphate Cell Test, Cat. No. 114543, 114729, and
100673 or Phosphate Test, Cat. No. 114848 in conjunction
with Crack Set 10/10C, Cat. No. 114687 resp. 114688 can
be used.