Pipette 5.0 ml of the
sample into a test tube.
Add 5 drops of PO
4
-1
and mix.
Measuring 0.05 – 5.00 mg/l PO
4
-P 0.2 – 15.3 mg/l PO
4
0.11 – 11.46 mg/l P
2
O
5
10-mm cell
range: 0.03 – 2.50 mg/l PO
4
-P 0.09 – 7.67 mg/l PO
4
0.07 – 5.73 mg/l P
2
O
5
20-mm cell
0.010 – 1.000 mg/l PO
4
-P 0.03 – 3.07 mg/l PO
4
0.02 – 2.29 mg/l P
2
O
5
50-mm cell
Expression of results also possible in mmol/l.
Reaction time:
5 minutes
Transfer the solution into
a corresponding cell.
Select method with
AutoSelector.
Place the cell into the
cell compartment
.
Important:
For measurement in the 50-mm cell, the sample volume
and the volume of the reagents hav
e to be doubled for
each.
Alternatively, the semi-microcell, Cat.No. 173502, can be
used.
For the determination of total phosphorus = sum of
orthophosphate, polyphosphate, and organophos-
phate a pretreatment with Crack Set 10C, Cat.No.
114688, or Crack
Set 10, Cat.No. 114687, and thermore-
actor
is necessary.
Result can be expressed as sum of phosphorus ( P).
114848
Test
Phosphate
Determination of orthophosphate
Add 1 level blue micro-
spoon of
PO
4
-2.
Shake vigorously to
dissolve the solid
substance.
Quality assurance:
To check the measurement system (test reagents,
measurement device, and handling) w
e recommended
to use Spectroquant
®
CombiCheck 10, Cat.No. 114676.
The data for the measurement in the 50-mm rectangular
cell are already programmed in the photometer.
Ready-for-use phosphate standard solution Certipur
®
,
Cat.No. 119898,
concentration 1000 mg/l PO
4
3–
, can also
be used after diluting accordingly.
To check for sample-dependent effects the use of addition
solutions (e.g. in CombiCheck 10) is highly recommended.
Check the pH of the
sample, specified range:
pH 0 – 10.
If required, add dilute
sulfuric acid drop by
drop to adjust the pH.
Release 06/2014 - Spectroquant
®
UV/VIS Spectrophotometer Pharo 300
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