Spectroquant Pharo 300 - Page 377

Pipette 8.0 ml of distilled
water (Water for analysis
EMSURE
®
, Cat.No.
116754, is recommend-
ed) into a test tube.
Add 0.50 ml of the
sample with pipette and
mix.
Measuring 1.0 –100.0 mg/l PO
4
-P 3 – 307 mg/l PO
4
2 – 229 mg/l P
2
O
5
10-mm cell
range: Expression of results also possible in mmol/l.
Shake vigorously to
dissolve the solid
substance.
Reaction time:
5 minutes
Transfer the solution into
a cell.
Select method with
AutoSelector.
100798
Test
Phosphate
Determination of orthophosphate
Add 0.50 ml of PO
4
-1
with pipette and mix.
Add 1 dose of PO
4
-2
using the blue dose-
metering cap.
Place the cell into the
cell compartment.
Quality assurance:
To check the measurement system (test reagents,
measurement device, and handling)
ready-for-use
phosphate standard solution Certipur
®
, Cat.No. 119898,
concentration 1000 mg/l PO
4
3–
, can be used after
diluting accordingly.
Check the pH of the
sample, specified range:
pH 0 – 10.
If required, add dilute
sulfuric acid drop by
drop to adjust the pH.
Release 06/2014 - Spectroquant
®
UV/VIS Spectrophotometer Pharo 300
Important:
For the determination of total phosphorus = sum of
orthophosphate,
polyphosphate and organophosphate
either Phosphate Cell Test, Cat. No. 114543, 114729, and
100673 or Phosphate Test, Cat. No. 114848 in conjunction
with Crack Set 10/10C, Cat. No. 114687 resp. 114688 can
be used.