Spectroquant
®
Pharo 300 Operation
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ba75703d07 04/2014
AutoCheck With the AutoCheck function the photometer checks and calibrates the opti-
cal measuring unit. The AutoCheck is automatically carried out if measure-
ment settings were changed since the last measurement, e.g.:
if a different wavelength was selected or
if a different method was selected.
If necessary, the photometer asks you to remove the cell from the cell shaft.
With unchanged measurement settings, the AutoCheck is carried out in the
background at regular intervals of 5 minutes. The AutoCheck can only be car-
ried out in the background if the cell shaft is empty. If a cell is in the cell shaft
the AutoCheck is carried out only after the cell was removed.
Note
Remove the cell from the cell shaft after every measurement. Thus the pho-
tometer can carry out the regular AutoCheck.
Cells must be completely removed from the cell shaft.
Cells that are removed only half disturb the AutoCheck measurement and, as
a consequence, falsify measured values until the next AutoCheck is carried
out.
Plastic cells that are not recognized by the automatic cell recognition also dis-
turb the AutoCheck.
Note
During a running kinetic measurement the photometer cannot carry out any
AutoCheck. That is why in this case a warm-up time of two hours is required.
After this time the signal is stable enough so that the measurement accuracy
is secured over a longer period of time.
Automatic
wavelength
calibration
With the automatic wavelength calibration function the photometer checks and cali-
brates the accuracy of the wavelengths created by the monochromator.
The wavelength calibration of the photometer is regularly carried out after switching
on (with the self-test) and is automatically repeated during operation after 15, 30, 60,
120 and 240 minutes.
A note is displayed while the photometer is carrying out the automatic wave-
length calibration. The automatic wavelength calibration only starts when the
cell shaft is empty.
If a cell is in the cell shaft the wavelength calibration is carried out only after
the cell was removed.
Display illumination The photometer automatically switches off the display illumination if no key
has been pressed for 5 minutes. The illumination is switched on again with
the next keystroke. The function of the key becomes active only with the fol-
lowing keystroke.
Switching off To switch the photometer off, keep the <ON/OFF> key depressed until the
photometer is switched off.