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Waters ACQUITY UPLC User Manual

Waters ACQUITY UPLC
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ACQUITY UPLC
Column Compartments Operators Overview
and Maintenance Information
Revision C
Copyright © Waters Corporation 20102011
All rights reserved

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Questions and Answers:

Waters ACQUITY UPLC Specifications

General IconGeneral
TechnologyUltra Performance Liquid Chromatography (UPLC)
Flow Rate Range0.001 to 2.000 mL/min
Maximum Pressure15, 000 psi (1, 034 bar)
Temperature Range4 °C to 65 °C
Data Sampling Rateup to 80 Hz
Detector OptionsPhotodiode Array (PDA), UV/Vis, Fluorescence, Evaporative Light Scattering
Detection Wavelength Range190 to 800 nm
Column CompatibilityUPLC Columns (1.7 μm particle size)

Summary

ACQUITY UPLC Column Compartments

Operator's Overview and Maintenance Information

Provides an overview of ACQUITY UPLC column compartments and maintenance procedures.

Copyright and Safety Notices

Specific Warnings for Column Compartments

Lists critical safety warnings related to column compartment usage, including solvent autoignition and hazardous materials.

System Overview

Column Compartment Types

Lists the different types of ACQUITY UPLC column compartments available for use.

Column Heater (CH-A) Description

Details the standard ACQUITY UPLC column heater (CH-A), its temperature range, and features.

Active Preheater Functionality

Column Heater Components

Major Components of the Column Heater

Identifies and describes the key components within the column heater assembly.

30-cm Column Heater (CH-30A)

Column Managers (CM-A and CM-Aux)

Column Manager Components

Details the components of the Column Manager (CM-A) and Auxiliary Column Manager (CM-Aux).

Column Manager Major Components

Column Manager Configurations

Auxiliary Column Manager Configurations

Explains configurations using CM-Aux modules for expanded column capacity.

ACQUITY UPLC 30-cm Column Heater/Cooler (CHC)

CHC Unit Features and Capabilities

Describes the features of the 30-cm column heater/cooler, including column capacity and mounting.

Preparing Column Compartments

Fitting Installation Recommendations

Provides guidelines for correctly installing fittings to prevent bandspreading and ensure proper connections.

Installing the Standard Column Heater

Installing the Optional MS Extension Bracket

Details the process for installing an MS extension bracket to position the column heater for mass spec applications.

Installing the Optional Column Stabilizer Assembly

Installing the 30-cm Column Heater

Moving the Preheater Key

Installing the Column Manager

Plumbing the Column Manager

Details on connecting the necessary tubing for column manager and auxiliary manager modules.

CM-Aux Module Installation

Single-Stack Configuration Setup

Guide to setting up a single-stack system with one CM-Aux module for up to four columns.

Repositioning System Instruments

Steps to rearrange instruments within the system stack for a new configuration.

Installing Extended Feet on Bottommost Module

Installing Two CM-Aux Modules

Multiple-Stack Configuration Steps

General steps to establish a multiple-stack configuration using two CM-Aux modules.

Removing Fittings from Valves

System Module Configuration Selection

Guidance on selecting a system module configuration based on instrument identification.

Exchanging CM-A Valve Cartridges

CM-A Inlet and Outlet Switching-Valve Cartridge Exchange

Steps to exchange the switching-valve cartridges for the CM-A module.

Tubing Installation and Diagrams

Installing the Magnetic Diagram

Instructions for installing compartment diagrams onto module doors.

Installing the Tubing

General steps and considerations for installing system tubing connections.

Column Configurations and Plumbing

Four-Column Configurations (Right-Hand Inlet)

Diagram and notes for a four-column setup with the inlet valve on the right.

Four-Column Configurations (Left-Hand Inlet)

Diagram and notes for a four-column setup with the inlet valve on the left.

Six-Column Configurations (Left-Hand Inlet)

Diagram and notes for a six-column setup with the inlet valve on the left.

Four and Six Column Plumbing Details

Configuration Plumbing Connection Details

Tables detailing tubing part numbers and lengths for four and six column configurations.

Identifying Switching Valves and Preheaters

Assembling New Fittings

Instructions for assembling new fittings, including marking compression screws and fitting bodies.

Installing Dual-Threaded Fittings

Configuring Instruments

Four-Column Configuration Setup

Steps to configure the system for a four-column setup using the ACQUITY UPLC Console.

Six-Column Configuration Setup

Steps to configure the system for a six-column setup using the ACQUITY UPLC Console.

Four-Column Configuration with 50mm Columns

Details on configuring a four-column setup using 50mm columns and CM-Aux modules.

Advanced Mode Operation

Explains how to use advanced mode for direct control of valves in the CM-A.

CM-A and CM-Aux LEDs

Power LED Status

Describes the meaning of the Power LED indicator on CM-A and CM-Aux modules.

Run LED Status

Explains the Run LED indicator, showing when the instrument is operating or idle.

Installing the Columns (ACQUITY UPLC 30-cm CHC)

Installing the Optional Column In-line Filter

Installing the Column (CH-A, CM-A, CM-Aux, and CH-30A)

Using the Column Compartments

Column Compartment Door Operation

Instructions on closing and securing the column compartment door, ensuring proper seal alignment.

ACQUITY UPLC 30-cm CHC Column Configurations

Activating Auxiliary Column Manager Units

Control Panels

Column Manager Control Panel

Describes the column manager control panel interface for monitoring and setting column temperature and selection.

Resolving Leak Sensor Errors

Resolving Column Compartment Leak Sensor Errors

Procedures for identifying and resolving leaks detected by column compartment sensors.

Maintaining the Column Compartments

Routine Maintenance Procedures

Overview of routine maintenance tasks recommended for column compartments.

Contacting Waters Technical Service

Information on how and when to contact Waters Technical Service for support.

Locating System Serial Numbers

Maintenance Schedule

Maintenance Considerations

Safety and Handling Precautions

Important safety and handling advisories to observe during maintenance operations.

Configuring Maintenance Warnings

How to set up maintenance counters and warnings for proactive component management.

Replacing the Column Heater's Leak Sensor

Replacing Column Leak Sensors

Replacing the 30-cm Column Heater's Leak Sensor

Information that only Waters Technical Service should replace the 30-cm column heater's leak sensor.

Replacing the Column Manager's Leak Sensor

Procedure for replacing the leak sensor in column manager or auxiliary column manager modules.

Replacing the Column Manager Switching-Valve Cartridge

Replacing the Column

Replacing the Active Preheater Assembly

Replacing the Column Stabilizer Assembly (CH-A only)

Replacing the Frit in the In-line Filter Unit

Replacing the Column Heater/Cooler Fuse

Cleaning the Instrument's Exterior

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