Chapter 2: BD FACSDiva Software Overview 39
Table 2-3 provides a description of each setting.
Table 2-3 HTS settings definitions
The Sample Flow Rate is the speed the syringe aspirates sample from the well in µL/
second.
To determine the acquisition time, divide the sample volume by the sample rate:
The Sample Volume is the volume of sample aspirated from each well for acquisition.
During acquisition in standard mode, BD HTS aspirates the selected sample
volume (2–200 µL) plus an additional 20 µL from the well. During acquisition
in high throughput mode, BD HTS aspirates a fixed 22 µL per well even though
you can select a sample volume between 2–10 µL.
Make sure each well contains sufficient sample for the entered volume plus the
dead volume. Insufficient volume can introduce air bubbles into the system.
!Tip To make sure you do not run out of sample, BD recommends that
you prepare your plate with a minimum of 250 µL of sample/well for a 96-well
plate in standard mode, 100 µL/well for a 96-well plate in high throughput
mode, and 50 µL/well for a 384-well plate (either mode).
The Mixing Volume is the volume of sample aspirated and dispensed during mixing.
Make sure each well on your plate contains sufficient sample for mixing.
Insufficient volume can introduce air bubbles into the system. BD recommends
a mixing volume that is one-half the available volume. See Understanding
Volumes on page 25 and Mixing on page 26.
Adequately mix the sample before pipetting it into the plate. Use the mixing
cycle only to maintain a homogeneous particle suspension.
The Mixing Speed is the speed that the syringe aspirates sample from and dispenses
sample to the well during mixing.
The Number of Mixes is the number of mixing cycles that are performed before a
sample is aspirated.
The Wash Volume is the volume of sheath fluid dispensed for rinsing between wells.
acquisition time (sec)
sample volume (µL)
sample rate (µL/sec)
-------------------------------------------------
=
To Next Page
Loading...
