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BD FACSCanto
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62 BD High Throughput Sampler User’s Guide
Figure 3-3 Installing the SIT protector
Slide the protector over the SIT, and push up on the tube retainer until you
can screw it onto the SIT. Tighten the tube retainer.
6 Attach the HTS sample coupler to the cytometer SIT.
Slide the sample coupler onto the SIT until you reach a hard stop. Make
sure the sample coupler tubing is not kinked or twisted. Hold the coupler
with one hand while you tighten the top nut with the other hand.
Note that there should be a gap between the tightening nut and the bottom
of the SIT protector (Figure 3-4 on page 63). If you don’t see a gap,
unscrew the tube retainer, push the SIT protector all the way up, and
retighten the tube retainer.
SIT protector
tube retainer

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