Spectroquant Pharo 300 - Page 242

Measuring 0.05 – 3.00 mg/l NH
4
-N 0.06 – 3.86 mg/l NH
4
10-mm cell
range: 0.05 – 3.00 mg/l NH
3
-N 0.06 – 3.65 mg/l NH
3
10-mm cell
0.03 – 1.50 mg/l NH
4
-N 0.04 – 1.93 mg/l NH
4
20-mm cell
0.03 – 1.50 mg/l NH
3
-N 0.04 – 1.82 mg/l NH
3
20-mm cell
0.010 – 0.500 mg/l NH
4
-N 0.013 – 0.644 mg/l NH
4
50-mm cell
0.010 – 0.500 mg/l NH
3
-N 0.013 – 0.608 mg/l NH
3
50-mm cell
Expression of results also possible in mmol/l.
Ammonium
114752
Test
Add 4 drops of NH
4
-3
and mix.
Reaction time:
5 minutes
Transfer the solution into
a corresponding cell.
Select method with
AutoSelector.
Pipette 5.0 ml of the
sample into a test tube.
Add 0.60 ml of NH
4
-1
with pipette and mix.
Add 1 level blue
microspoon of NH
4
-2.
Shake vigorously to
dissolve the solid
substance.
Reaction time:
5 minutes
Check the pH of the
sample, specified range
:
pH 4 – 13.
If required, add dilute
sodium hydroxide
solution or sulfuric acid
drop by drop to adjust
the pH.
Release 06/2014 - Spectroquant
®
UV/VIS Spectrophotometer Pharo 300
Place the cell into the
cell compartment.
Important:
Very high ammonium concentrations in the sample
produce turquoise-colored solutions (measurement solu-
tion should be yellow-g
reen to green) and false-low read-
ings are yielded. In such cases the sample must be di-
luted (plausibility check).
To measure in the 50-mm cell, the sample volume and the
volume of the reagents have to be doubled for each.
Alternatively, the semi-microcell, Cat.No. 173502, can be
used.
Quality assurance:
To check the measurement system (test reagents, meas-
urement device, and handling) w
e recommended to use
Spectroquant
®
CombiCheck 50, Cat.No. 114695, or the
Standard solution for photometric applications, CRM,
Cat.No. 125022, 125023, and 125024.
Ready-for-use ammonium standard solution Certipur
®
,
Cat.No. 119812,
concentration 1000 mg/l NH
4
+
, can also
be used after diluting accordingly.
To check for sample-dependent effects the use of addition
solutions (e.g. in CombiCheck 50) is highly recommended.