Spectroquant Pharo 300 - Page 243

Ammonium
100683
Test
Pipette 5.0 ml of NH
4
-1
into a test tube.
Add
0.10 ml of the
sample
with pipette.
Check the pH of the
sample, specified range
:
pH 4 – 13.
If required, add dilute
sodium hydroxide
solution or sulfuric acid
drop by drop to adjust
the pH.
Important:
Very high ammonium concentrations in the sample
produce turquoise-colored solutions (measurement solu-
tion should be yellow-g
reen to green) and false-low read-
ings are yielded. In such cases the sample must be di-
luted (plausibility check).
Release 06/2014 - Spectroquant
®
UV/VIS Spectrophotometer Pharo 300
Continue as mentioned above; starting
from the addition of NH
4
-2 (Fig. 4). Select
method with AutoSelector measuring range
5 – 150 mg/l NH
4
-N.
Quality assurance:
To check the measurement system (test reagents, meas-
urement device, and handling) w
e recommended to use
Spectroquant
®
CombiCheck 70, Cat.No. 114689, or the
Standard solution for photometric applications, CRM,
Cat.No. 125025, 125026, and 125027.
Ready-for-use ammonium standard solution Certipur
®
,
Cat.No. 119812,
concentration 1000 mg/l NH
4
+
, can also
be used after diluting accordingly.
To check for sample-dependent effects the use of addition
solutions (e.g. in CombiCheck 70) is highly recommended.
Measuring range: 2.0 – 75.0 mg/l NH
4
-N 2.6 – 96.6 mg/l NH
4
10-mm cell
5 – 150 mg/l NH
4
-N 6 – 193 mg/l NH
4
10-mm cell
2.0 – 75.0 mg/l NH
3
-N 2.4 – 91.2 mg/l NH
3
10-mm cell
5 – 150 mg/l NH
3
-N 6 – 182 mg/l NH
3
10-mm cell
Expression of results also possible in mmol/l.
Transfer the solution
into a cell.
Select method with
AutoSelector measuring
range 2.0 – 75.0 mg/l
NH
4
-N.
Place the cell into the
cell compartment.
Pipette 5.0 ml of NH
4
-1
into a test tube.
Add
0.20 ml of the
sample with pipette
.
Add 1 level blue micro-
spoon of NH
4
-2.
Shake vigorously to
dissolve the solid
substance.
Reaction time:
15 minutes
Measuring range: 2.0 – 75.0 mg/l NH
4
-N
Measuring range: 5 – 150 mg/l NH
4
-N
Check the pH of the
sample, specified range
:
pH 4 – 13.
If required, add dilute
sodium hydroxide
solution or sulfuric acid
drop by drop to adjust
the pH.