ZEISS OPERATION Axio Imager 2
Illumination and contrast methods
170 430000-7544-001 01/2016
The DIC method employs polarized light and therefore is disturbed by birefringent objects, such
as films, in the light path between polarizer and analyzer which are occasionally used in
conjunction with histological sections. The same applies to acrylic glass culture chambers, if the
chamber bottom is made of plastic. In these cases, it is advisable to use chambers with bottom
plates of glass to avoid loss of optical performance.
5 Sliding button for aperture diaphragm
6 Condenser with DIC prism and polarizer
Fig. 197 Components required for transmitted-light DIC
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