Chapter 7: Running Samples with BD FACSDiva Software 123
7 Create a statistics view; edit the view to show the Lymphocyte population
and subpopulations, and to display the mean for all fluorochromes.
To create a statistics view, right-click any plot and choose Create Statistics
View. The resulting statistics view lists the number of events, %Parent, and
means of the plot parameters for all populations displayed in the plot.
8 Draw a gate around the CD19 positive population on the CD3 APC vs
CD19 PE-Cy7 plot; name the population B Cells.
9 Draw a gate around the CD16+56–positive population on the CD3 APC vs
CD16+56 PE plot; name the population NK Cells.
10 Draw a gate around the double-positive population on the CD3 APC vs
CD4 APC-Cy7 plot, and name the population T Helper.
11 Draw a gate around the double-positive population on the CD3 APC vs
CD8 PerCP-Cy5-5 plot; name the population T Cytotoxic.
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