148 BD FACSCanto II Instructions for Use
5 When prompted, install a tube with 3 mL of BD FACSClean solution or
10% bleach on the SIT. Firmly push up on the tube until it comes to a
complete stop and is fully seated.
6 Click .
7 After 5 minutes, click .
Do not click .
8 When prompted, unload the tube.
9 Install a tube with 3 mL of DI water. Firmly push up on the tube until it
comes to a complete stop and is fully seated.
10 Repeat steps 6 through 8.
11 Choose File > Exit, and select Run fluidics shutdown and exit.
BD Biosciences recommends turning off the cytometer when not in use to
preserve laser life.
NOTICE After every 4 hours of operation, and during cytometer
shutdown, you will hear a hiss when condensed water discharges from the
fluidics cart pumps into the condensation trap.
12 When fluidics shutdown completes, the software closes.
13 Turn off cytometer power.
To prevent fluid overflow, do not leave a tube on the SIT during fluidics
shutdown.
To Next Page
Loading...
