236 BD FACSCanto II Instructions for Use
These lasers are contained within the cytometer, therefore the BD FACSCanto II
flow cytometer is a Class I (1) laser product.
Excitation Optics
Emission Optics
Optical platform Fixed optical assembly
Beam geometry (all lasers) 9 μm x 65 μm elliptical beam
Collection lens Optical gel–coupled to flow cell
Numerical aperture (NA) = 1.2
Fluorescence detection 6 to 8 photomultiplier tube detectors:
Wavelength ranges detected from 488-nm laser:
• 750–810 nm (PE-Cy7)
• 670–735 nm (PerCP-Cy5.5)
• 610–637 nm (PE-Texas Red
®, optional)
• 564–606 nm (PE)
• 515–545 nm (FITC)
Wavelength ranges detected from 633-nm laser:
• 750–810 nm (APC-Cy7)
• 701–723 nm (Alexa Fluor
® 700, optional)
• 650–670 nm (APC)
Wavelength ranges detected from 405-nm laser:
• 502–535 nm (AmCyan)
• 425–475 nm (Pacific Blue™)
Forward scatter detection Photodiode with 488/10 bandpass filter
Side scatter detection PMT with 488/10 bandpass filter
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