BD FACSCanto II - Page 244

244 BD FACSCanto II Instructions for Use
Experiment Layout window 116
Experiments
immunophenotyping 116
sample optimization 78
F
FCS files
adding to worklist 109
not created 208
files
optimized setup 69, 70
process control 92
filters
air, replacing 161
bleeding 158
changing 162
fluidic 22
purging 158
waste container cap 157
flow cell 17
about 17
access door 15
checking for bubbles 48
problems 190, 191
flow rates 237
appropriate 118
fluid
leaks
cytometer 194
fluidics cart 198
SIT 193
levels 47
not recommended 172
required 28
fluidics
level
indicators 47
sensors, replacing 183
long clean 160
pressure errors 206
priming 169
fluidics cart
components 22
controls 24
dimensions 238
fuses, replacing 186
maintenance 156, 166
reconnecting tubing 181
troubleshooting 193, 198
fluids
leaks
fluidics cart 225
fluorescence
detection 236
properties, particle 17
signal, troubleshooting 208, 221
focal lengths
barcode reader 42
forward scatter (FSC)
diode 17
fuses, replacing 186
G
gates, adjusting 102
gating
compensation tubes 87
data 116
manually adjusting 102
reapplying auto-gates 104
granularity, particle 17
H
hazard symbol definitions xii