246 BD FACSCanto II Instructions for Use
optics
access door 15
filters and mirrors 18–19
octagon 18–19
trigon 18–19
optimization settings file 69, 70
optimizing during acquisition 97, 100
P
panels, choosing 91
parameters
adding or deleting 80
changing 80
labels 116
plot 97
scatter, distorted 222
particle
fluorescence properties 17
granularity 17
shape 17
size 17
photodiode 17
photomultiplier tubes (PMTs), applying
voltages 83
plenum 16
plots
adjusting gates 102
excessive debris 208, 222
Multitest examples 213
no events 220, 221
parameters 97
troubleshooting 213
populations, troubleshooting 223
power button 15
preferences
mixing 136
user 126
pressure
errors, tube 202
fluidics 206
priming, fluid lines 169
printing setup reports 69
process controls 92
purging filters 158
Q
QC
cytometer 50
messages 210
quality control (QC) 50
R
rack, carousel 108, 239
recording
compensation tubes 87
data 118
removing
carousel rack 137
tubes 59
replacing
air filter 161
fuses 186
reports
Carousel 133
Lab
reviewing 101, 105
viewing 97, 111
printing automatically 141
setup 69
requirements, system 28
re-running
samples 141
tubes 104
results, unexpected 222
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