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BD FACSCanto II
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vi BD FACSCanto II Instructions for Use
Chapter 4: Starting Up 45
Chapter 5: Cytometer QC and Setup 49
Performing Automated Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
Running Setup Using Manual Loading . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Running Setup Using the Loader . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
Optimizing with BD FACSCanto Clinical Software . . . . . . . . . . . . . . . . . . . . 64
Maintaining User-Specific Optimization Settings . . . . . . . . . . . . . . . . . . 70
Using Cytometer Controls . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
Reviewing Levey-Jennings Reports . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
Optimizing with BD FACSDiva Software . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
Verifying Cytometer Configuration and User Preferences . . . . . . . . . . . . 77
Creating the Experiment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
Applying the Setup Results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81
Creating Compensation Controls . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82
Optimizing Cytometer Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
Calculating Compensation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87
Chapter 6: Running Samples with BD FACSCanto Clinical Software 89
Running an Acquisition Worklist . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90
Entering Information into a Worklist . . . . . . . . . . . . . . . . . . . . . . . . . . . 91
Running a Process Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92
Acquiring Samples . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93
Options During Acquisition . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 99
Importing a Worklist from SPA Software . . . . . . . . . . . . . . . . . . . . . . . . 107
Reviewing an Analysis Worklist . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 108
Creating a New Analysis Worklist . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 108
Reviewing a Worklist . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111
Logging Out . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113

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