Chapter 5: Cytometer QC and Setup 63
11 Wait for setup to finish.
The software adjusts cytometer settings to place the beads on scale. Note
that it is normal for the beads to move to the baseline and back on scale
during this process.
12 Decide what to do next:
• If Setup is unsuccessful or if setup results are out of range, do not click
Finish. Note the message provided by the software (see Figure 5-5 on
page 60) and consult BD FACSCanto Clinical Software
Troubleshooting on page 199.
• If setup is successful, see Table 5-1 on page 60.
13 It is important to monitor setup data for trends. See Reviewing Levey-
Jennings Reports on page 74.
14 If you are using BD FACSDiva software to acquire samples, close
BD FACSCanto clinical software.
Incorrect values are saved if you accept a setup that is unsuccessful or setup
results that are out of range.
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