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Zeiss Axio Imager 2 - Page 188

Zeiss Axio Imager 2
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ZEISS OPERATION Axio Imager 2
Illumination and contrast methods
188 430000-7544-001 01/2016
Place the specimen on the stage, focus on it
and rotate the control wheel (Fig. 2061) on the
CDIC slider 6x20 or the modulator turret
(Fig. 207/1) until the specimen detail of interest
is visible at maximum contrast. Rotating the
stage is no longer necessary. When both line
marks face each other (Fig. 207/3), this
corresponds to the mid-position. 45° rotation in
both directions is possible.
The contrast can be optimized by turning the
setscrew (Fig. 206/2) on the C-DIC slider or on
the modulator turret (Fig. 207/2). When the
triangular and line marks face each other
(Fig. 207/3), this corresponds approximately to
the extinction position (best contrast).
(5) Setting the reflected light DIC with
motorized four-position modulator turret for
circular DIC/TIC
Prepare the microscope for reflected-light
brightfield.
Bring the C DIC P&C reflector module into the
light path.
On the nosepiece, swivel in the objective
suitable for DIC.
On the modulator turret, swivel in the
appropriate DIC prism by pressing button
(Fig. 208/1) for forward rotation or on button
(Fig. 208/2) for backward rotation of the turret.
Place the specimen on the stage, bring it into
focus and turn knob (Fig. 208/2 SHIFT) so that
the structure of interest can be seen in
maximum contrast.
The contrast can be optimized by turning knob
(Fig. 208/1 - AZIMUTH) on the modulator
turret.
Fig. 207 4-position modulator turret
Fig. 208 Motorized four-position modulator
turret for circular DIC/TIC

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