114 Agilent 7100 Capillary Electrophoresis System User Manual
5 Using the Agilent 7100 Capillary Electrophoresis System
Capillary Electrophoresis, CE
1 Make sure the buffer is filtered; use a 0.25 µm filter. For buffers with a high
viscosity, a larger pore size, for example, 0.45 µm is also suitable.
2 Fill the electrolyte bottle with buffer.
For 500 mL bottles (part number 9300-1748), use a minimum of 100 mL to
make sure the frit is submerged in buffer. Use a maximum of 400 mL. For
100 mL bottles (part number 5042-6478), use a minimum of 40 mL to make
sure the frit is submerged in buffer. Use a maximum of 80 mL.
3 Tighten the cap.
Make sure the cap is tightened firmly, as pressure will be applied to the
bottle.
4 Choose OK in the Change Bottles dialog box.
Wait until the pressure has built up again, that is the instrument reaches
the ready state.
The right-hand bottle is the waste bottle. It is used to store buffer emptied
from vials in the tray. Empty the bottle whenever you refill the electrolyte
bottle, following the same procedure as described above. Empty the bottle
frequently. The error message "Waste Bottle Full" appears if the waste bottle is
full.
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