Agilent 7100 Capillary Electrophoresis System User Manual 221
Maintenance 8
Maintenance of the Replenishment System
A cycle consists of:
1 Submerging the short needle into the cleaning liquid and briefly sucking
cleaning liquid into the short needle.
2 Removing the short needle from the cleaning liquid, and sucking a slug of
air into the short needle.
The alternate slugs of liquid and air exit the level sensing tubing into the waste
bottle. The main screen reply line counts the cycles as they take place. You can
check for proper function afterwards with the IRP level sensing test from the
injection replenishment system tests or by performing buffer replenishment in
a vial.
This function is designed to prevent a gradual build-up of buffer crystals. Large, solid plugs
of precipitate which have been allowed to form in the short needle may not be removable
using this function. In such cases, we recommend exchanging the restrictor capillaries and
or the replenishment needle by personnel trained by Agilent Technologies.
If the replenishment system will not be used for some time the level sensor should be
cleaned with water. Subsequently the replenishment system should be cleaned with water
and then with isopropanol by the Flush Tubes and Clean Tubes operations.
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