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Agilent Technologies 7100 User Manual
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©
Agilent T
echnologies 2009
Printed in Germany
12/2009
*G7100-90000*
*G7100-90000*
G7100-90000
www
.agilent.com
Agilent T
echnologies
In This Book
This manual cont
ains technical
re
fe
r
en
ce
i
n
for
m
at
i
on
a
bo
u
t
t
he
Agilent 7100 Capillar
y
Electrophoresis Syst
em.
The manual descri
bes the
f
ollo
wing:
• introduction and specifications,
• inst
allation,
• using and optimizing,
• troubleshooting,
• maintenance
• par
ts identification,
• saf
ety and related information.
265
Table of Contents
English
5
Default Chapter
5
Table of Contents
5
Introduction
13
Introduction to the Agilent 7100 Capillary Electrophoresis System
14
Getting Help
14
Where to Find Information
15
What Learning Products Are Available
16
Site Requirements and Specifications
17
Site Requirements
18
Power Consideration
18
Power Cords
19
Bench Space
20
Environment
20
Physical Specifications
21
Performance Specifications
22
Anforderungen an den Aufstellungsort
23
Stromversorgung
23
Netzkabel
24
Platzbedarf
25
Umgebungsbedingungen
25
Abmessungen und Gewicht
26
Leistungsspezifikationen
27
Exigences D'installation
28
Remarques À Propos de L'alimentation
28
Câbles D'alimentation
29
Encombrement
30
Environnement
30
Caractéristiques Physiques
31
Caractéristiques de Performance
32
Requisiti Ambientali
33
Alimentazione
33
Cavi DI Alimentazione
34
Spazio Necessario
35
Ambiente
35
Specifiche Fisiche
36
Specifiche Delle Prestazioni
37
Requisitos de las Instalaciones
38
Consideraciones sobre la Corriente
38
Cables de Alimentación
39
Espacio en el Banco
40
Entorno
40
Especificaciones Físicas
41
Especificaciones de Rendimiento
42
設置について
43
電源について
43
電源コード
44
作業台スペース
45
環境条件
45
物理的仕様
46
性能仕様
47
场地要求
48
电源要求
48
电源线
49
工作台位置
50
物理规格
51
性能规格
52
Installing the Instrument
53
Unpacking the Agilent CE Instrument
54
Damaged Packaging
54
Unpacking the CE Instrument
54
Delivery Checklist
55
Installing the Instrument
57
Physical Connection
57
LAN Configuration
61
What You Have to Do First
62
TCP/IP Parameter Configuration
63
Configuration Switch
64
Initialization Mode Selection
65
Link Configuration Selection
69
Storing the Settings Permanently with Bootp
70
Manual Configuration
71
With Telnet
72
Agilent Bootp Service
75
Location of the Media Access Control (MAC) Address
76
Using the Agilent 7100 Capillary Electrophoresis System
77
System Definition
78
Controlling the Agilent CE Instrument
79
Before You Start the Agilent CE Instrument
79
Starting up the Agilent CE Instrument
79
Starting Your Computer and the Agilent Chemstation Software
81
Starting the Agilent Chemstation Software the First Time
82
Working with the CE Diagram
84
Common Software Toolbar
84
Software Toolbar
85
Graphical User Interface Explanation
86
Menus in the CE and DAD Diagram
88
System Vialtable
90
How to Prepare Your Agilent CE Instrument for Analysis
91
Capillary Electrophoresis, CE
92
How to Use Your Agilent CE Instrument for Capillary Electrophoresis Separations
92
Agilent CE Method Parameters
92
Vials
96
Cassette Temperature
97
High Voltage System
98
Posttime and Stoptime
100
Replenishment
100
Preconditioning
101
Injection
103
Timetable
105
Detector Parameters
107
Switching the Lamp on
110
Preparing the Replenishment System if Needed
111
Preparing Vials for Buffer and Sample
115
Installing a Capillary in the Alignment Interface
124
Installing a Capillary in an Empty Capillary Cassette
128
Inserting the Cassette
132
Fraction Collection
135
Capillary Electrophoresis, Plus High Pressure Ce+P
136
Requirements
136
Outlet Lift Connection to the Tubing System
138
Preparing the Agilent CE Instrument for High Pressure Use
140
Operation and Function in Ce+P Mode
143
Injection Options with Ce+P
146
Timetable Options with Ce+P
146
Ce+P Control Using the CE Diagram
147
Capillary Electrochromatography, CEC
148
How to Use Your Agilent CE Instrument for Capillary Electrochromatography
148
Preparing the Agilent CE Instrument for High Pressure Use
150
CEC-Specific GUI Explanation
153
Injection Options with CEC
156
Timetable Options with CEC
157
CEC Control Using the CE Diagram
158
Running the CEC Analysis
159
Troubleshooting and Diagnostics
161
CE Problem Solving
162
Power on Fail
162
Leaks
163
Blocked Replenishment Needle
165
Leakage Current
165
Broken Capillary
166
Contaminated Insulation Plate
166
Empty Injection Vial
167
Dirt in the Vials
167
Air Bubble in Injection Vial or Run Buffer Vials
167
Air Bubble in Capillary
167
Problems with the Replenishment System and Buffers Containing SDS
168
Agilent Lab Advisor Software
169
Configuration
169
Hardware Information
171
Identifying Instrument Components
172
7100 Capillary Electrophoresis Instrument Electronics
173
Firmware
176
Resident System
176
Main System
176
Firmware Updates
177
Diode Array Detector
179
DAD Control
180
Digital to Analog Converter
181
Changing the Fuses for the Power Supplies
182
Installing Drainage Tubing
183
External Water Bath for Tray Cooling (Optional)
184
Analog to Digital Converter
186
Maintenance
189
Standard Operating Procedures for Maintenance of Your Agilent CE Instrument
190
Overview of Maintenance
191
Early Maintenance Feedback (EMF)
194
EMF Counters
194
Using the EMF Counters
194
Setting the EMF Limits
195
Cleaning the Electrodes, Pre-Punchers and Insulation Plate
196
Prepare the Agilent CE Instrument
197
Remove the Covers
198
Accessing the Electrodes
199
Accessing the Liquid Handling Module
201
Accessing and Removing the Pre-Punchers
203
Cleaning the Electrodes
205
Cleaning the Pre-Punchers
206
Cleaning the Insulation Plate
207
Reinstalling the Pre-Punchers
208
Reinstalling the Electrodes and the Insulation Plate
210
Reinstall the Capillary Cassette
215
Reinstall the Front Cover
215
Maintenance of the Replenishment System
216
Changing the Buffer Composition in the Replenishment System
217
The Replenishment System will Not be Used for some Time
218
Further Action
219
Operations for Replenishment Maintenance
219
Changing the Air Inlet Filter
222
Accessing the Air Filter
222
Changing the Lamp
224
Removing the Existing Lamp
225
Inserting a New Lamp
227
Further Action
227
Cleaning the Instrument
228
Parts and Materials for Maintenance
229
Overview of Maintenance Parts
230
Deutsch
231
Appendix
231
General Safety Information
232
Operation
232
Allgemeine Sicherheitsinformation
235
Informations Générales de Sécurité
238
Informazioni Generali Sulla Sicurezza
241
Información de Seguridad
244
安全に関する一般的な情報
247
一般安全信息
250
Setting up a Test Sample Run
252
Method Parameter CE (Default Settings)
253
Method Parameter DAD
255
Chemical and Biological Safety
256
The Waste Electrical and Electronic Equipment (WEEE) Directive (2002/96/EC)
257
Radio Interference
258
Sound Emission
259
Legal Notice
260
Agilent Technologies on Internet
261
Index
263
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Agilent Technologies 7100 Specifications
General
Brand
Agilent Technologies
Model
7100
Category
Laboratory Equipment
Language
English
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