D AssayMAP Bravo Platform
Bravo 96AM Head overview
202 Bravo Platform User Guide
Liquid-handling modes
The Bravo 96AM Head features include the ability to:
• Transfer liquids directly with the bare probes, through AssayMAP Bravo cartridges
mounted on the probes, and using mounted 250-µL large-transfer (LT) pipette tips
• Mount or remove the cartridges while holding liquid in the syringes
• Provide precise control of flow rates and volume required for moving fluid through
the packed resin in the cartridges
• Access 96-well and 384-well microplates
• Perform 96-channel liquid handling or partial-head liquid handling using a subset of
the 96 channels
Protocols for the Bravo 96AM Head can employ a combination of the following liquid-
handling modes:
• Bare probes
• AssayMAP Bravo cartridges mounted on the probes
• 250-µL LT pipette tips mounted on the probes
Capabilities of each liquid-handling mode
The following figure shows a side-by-side comparison of a bare probe, a probe with a
mounted cartridge, and a probe with a mounted 250-µL LT tip. The following table
provides the details for each.
Figure
Comparison of probe, probe with cartridge, and probe with 250-µL LT tip
Item Mode Description
1 Bare probe • Maximum volume: 250 µL can be aspirated into the
syringe before the syringe is full.
• Only 96-channel (full head) liquid handling is supported.
• As the tips of the syringes, the probes can be in direct
contact with the samples for liquid-handling tasks.
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