D AssayMAP Bravo Platform
Installing and using the Bravo 96AM Head
208 Bravo Platform User Guide
Installing and using the Bravo 96AM Head
This topic addresses the following:
• Guidelines for using and storing the Bravo 96AM Head
• About the tip box stripper pins
• Uninstalling the Bravo 96AM Head
• Installing the Bravo 96AM Head
Guidelines for using and storing the Bravo 96AM Head
Ensure that you understand the following warnings and cautions about the proper way
to handle and use the Bravo 96AM Head.
The probes of the Bravo 96AM Head are sharp and can scratch you if they brush
across your hand. A probe scratch can expose you to any contaminants remaining on
the probes. Be careful to avoid touching the probes.
Extended use of the Bravo 96AM Head with dry syringes for disposable-tip liquid
handling can shorten the life of the syringe seals.
To prevent potential damage, ensure the Bravo 96AM Head probes, pipette tips, or
cartridges do not touch the bottom of the 96AM Wash Station. To adjust the distance
from the bottom, you can use the Bravo liquid-handling task parameters in the
VWorks software.
Before leaving the Bravo 96AM Head idle overnight, wash the syringes thoroughly
with deionized water to prevent salt buildup that could damage the syringe seals.
To aspirate more than 140 µL of liquid, use the bare probes instead of disposable
pipette tips. The syringes can hold up to 250 µL. If you aspirate more than 140 µL using
250-µL pipette tips, the excess aspirated liquid will enter the syringes. Ensure that you
wash the syringes as appropriate for your protocol to prevent contamination.
For information about using a subset of the 96 channels, see “Requirements for partial-
head liquid handling” on page 219.
About the tip box stripper pins
If the Bravo 96AM Head tip box stripper pins are extended, the pins will crash into the
wash station during syringe washing. To prevent potential equipment damage,
ensure that the pins are always retracted during use.
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