D AssayMAP Bravo Platform
Bravo 96AM Head overview
204 Bravo Platform User Guide
Figure
Syringe-and-probe liquid-handling modes (cutaway views)
• Cartridge aspirate. A series of different liquids can be pulled up through the
cartridge bed in sequence without removing the mounted cartridges and washing
out the syringes in between the different aspirate steps.
• Cartridge dispense. Cartridges can be mounted while the syringes contain liquids.
The liquid in the syringes can be dispensed down through the mounted cartridges
at precisely controlled flow rates for different types of tasks.
For example, you can dispense equilibration buffer down through the mounted
cartridges for priming and equilibration tasks, and you can dispense elution buffer
through the mounted cartridges to recover eluate in a microplate.
• Direct probe. Liquid can be aspirated directly into the syringes and transferred using
the bare probes.
The cartridges can be removed to dispense the liquid collected in the syringes,
wash the syringes, and then aspirate other fluids into the syringes before mounting
the cartridges again for additional dispensing steps.
• Disposable pipette tip. As the preceding figure shows, the probe protrudes into the
pipette tip volume that is normally used to hold liquid. Therefore, the pipette tip
volume capacity is reduced when mounted on a probe.
Related information
Syringe
plunger
Cartridge
aspirate
Cartridge
dispense
Direct
probe
Disposable
tip
Probe
Cartridge
Inlet seal
5 µL bed
For more information about… See…
AssayMAP Bravo cartridges “AssayMAP cartridge overview” on
page 205
Bravo 96AM Head • “Bravo 96AM Head overview” on
page 200
• “Installing and using the Bravo 96AM
Head” on page 208
• “Requirements for partial-head liquid
handling” on page 219
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